High-Resolution RNA Structural Analysis via Cryo-EM

Written By Dina Hussein

Client

Stanford University, USA

Duration

2 Years

Technologies

  • Cryo-Electron Microscopy (Cryo-EM)

  • RNA Biochemistry

  • Vitrobot & Jet Vitrification

  • Gaussian-Based Data Modeling

  • 3D Structural Reconstruction

Project Overview

This project addressed one of the major challenges in structural biology: obtaining high-resolution Cryo-EM structures of RNA molecules. By integrating advanced RNA biochemistry, optimized sample preparation, and computational analysis, Bioheptaxia developed a robust workflow that significantly improved RNA imaging quality and reproducibility.

Scope of Work

  • Designed and implemented a complete RNA sample preparation workflow.

  • Performed in vitro transcription and high-purity RNA purification.

  • Optimized vitrification protocols using automated Cryo-EM systems.

  • Applied Gaussian-based modeling to improve structural interpretation.

  • Conducted Cryo-EM data collection and high-resolution 3D reconstruction.

Innovation

The project combined experimental molecular biology with advanced computational analysis to establish reproducible RNA Cryo-EM workflows, enabling laboratories to generate high-quality RNA structural datasets with greater efficiency and reliability.

Results

  • High-resolution RNA structural datasets.

  • Reproducible Cryo-EM preparation protocols.

  • Optimized computational analysis workflows.

  • Scalable methodology for future RNA structural studies.

Technical Impact

The developed workflow enabled research laboratories to transition from protein-focused Cryo-EM methodologies to RNA-compatible high-resolution pipelines through protocol optimization, technical training, and advanced data acquisition strategies.

Dina Hussein
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